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Bacillus clausii MB9 from the east coast regions of India Isolation, biochemical characterization and antimicrobial potentials
By: Devi, N. K. Asha.
Contributor(s): Balakrishnan, K | Gopal, R.
Subject(s): Antagonistic activity | Bacillus clausii | microorganisms | secondary metabolites In: Current Science 95(5)Summary: 91 bacterial colonies were isolated from water samples of 5 coastal regions of southern India (Tamil Nadu). Among these, 33% of the isolates (n=30) exhibited strong batericidal & fungicidal properties against pathogens. One promosing strain, designated as MB9, with strong antimicrobial activity against most of the pathogends tested was selected for further studies. Morphological, cultural, physiological, biochemical properties & molecular characterization, 16S rRNA sequencing & BLAST analysis (IMTECH, Chandigarh) indicated this strain as Bacillus clausii (100% similarity with GenBank sequences). The secondary metabolite (culture supernatant) produced by B. clausii MB9 at different salinities, temperatures & pH values are found to be stable at elevated temperature conditions. Moreover, the cutrue supernatant from this novel isolate remains active & retains its antimicrobial activities even after the supernatant was subjected to autoclaving temperatures (121 degree Celcius). There was no significant loss of antifungal activity after treatment with various detergents. The results obtained in the present investigation indicate that B. cluasii MB9 produced a novel, highly thermostable secondary metabolite showing a wide specturm of antimicrobial activities. Studies relating to the use this isolate as probiotics & also for the production of biocontrol agents (insect & mosquito larvicides), industrial enzymes (for leather dehairing & as a component of detergent) & molecular characterization of the active principle in the supernatant are in progress.| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
| Journal | Indira Gandhi Rashtriya Manav Sangrahalaya | Available |
91 bacterial colonies were isolated from water samples of 5 coastal regions of southern India (Tamil Nadu). Among these, 33% of the isolates (n=30) exhibited strong batericidal & fungicidal properties against pathogens. One promosing strain, designated as MB9, with strong antimicrobial activity against most of the pathogends tested was selected for further studies. Morphological, cultural, physiological, biochemical properties & molecular characterization, 16S rRNA sequencing & BLAST analysis (IMTECH, Chandigarh) indicated this strain as Bacillus clausii (100% similarity with GenBank sequences). The secondary metabolite (culture supernatant) produced by B. clausii MB9 at different salinities, temperatures & pH values are found to be stable at elevated temperature conditions. Moreover, the cutrue supernatant from this novel isolate remains active & retains its antimicrobial activities even after the supernatant was subjected to autoclaving temperatures (121 degree Celcius). There was no significant loss of antifungal activity after treatment with various detergents. The results obtained in the present investigation indicate that B. cluasii MB9 produced a novel, highly thermostable secondary metabolite showing a wide specturm of antimicrobial activities. Studies relating to the use this isolate as probiotics & also for the production of biocontrol agents (insect & mosquito larvicides), industrial enzymes (for leather dehairing & as a component of detergent) & molecular characterization of the active principle in the supernatant are in progress.