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A Simplified procedure for Isolation of Highly Purified Genomic DNA
By: Bardhan, Smriti.
Contributor(s): Sharan, Chakradhari | Singh, Dharmdeo N.
Subject(s): DNA | Hemoglobin | Polymerase Chain Reaction | Southern Hybridization | Medical Genetics In: Bionature 12(1-2)Summary: A simple and rapid procedure for the extraction of genomic DNA involving sodium perchlorate exposure and chloroform extractions followed by SDS and Pronase E treatment is described. The method can be completed within 8 hours. Variwty of tissues can be used as a source of genomic DNA(ie. blood, amniotic fluid, tissue culture, cord blood and organ tissue) and DNA yields of 250-300 (ug/ml per 5ml of blood with OD 260/OD 280 ratios of 1.8 can be routinely obtained with this procedure. Agarose gel electrophoresis of the extracted DNA shows a high molecular weight species which can be directly digested with a battery of restriction wnzymes. We also show that these DNA preparations can be used as templates in the polymerase chain reaction for an efficient amplification of desired DNA regions.| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
| Journal | Indira Gandhi Rashtriya Manav Sangrahalaya | Available |
A simple and rapid procedure for the extraction of genomic DNA involving sodium perchlorate exposure and chloroform extractions followed by SDS and Pronase E treatment is described. The method can be completed within 8 hours. Variwty of tissues can be used as a source of genomic DNA(ie. blood, amniotic fluid, tissue culture, cord blood and organ tissue) and DNA yields of 250-300 (ug/ml per 5ml of blood with OD 260/OD 280 ratios of 1.8 can be routinely obtained with this procedure. Agarose gel electrophoresis of the extracted DNA shows a high molecular weight species which can be directly digested with a battery of restriction wnzymes. We also show that these DNA preparations can be used as templates in the polymerase chain reaction for an efficient amplification of desired DNA regions.